Head of Lab: Prof. Ludomira Granicka, PhD, DSc


Magdalena Antosiak-Iwańska, PhD Eng.
Monika Drabik, PhD Eng.
Ewa Godlewska
Anna Grzeczkowicz, PhD
Angelika Kwiatkowska, PhD Eng.
Agata Lipko, PhD

Encapsulation of biological material within the membranes receives a great deal of interest due to possibility of biotechnological and biomedical applications, such as replacement, repair, or supporting the function of diseased tissue. The subject of our interest is approach to immobilization within the membranes of the biologically active material in order to obtain biological processes regulatory systems. Among the techniques used by us macroencapsulation, microencapsulation and nanoencapsulation can be mentioned.
Our research encompass immunoisolation problems as well as construction of the systems for anti-cancer therapy. This involves the membrane systems fabrication, the development of methods of membranes modifications, membrane properties evaluation with particular regard to the biocompatibility using the standardized by us methods, and in vivo verification of produced systems. Recent research program concerned the problem of effective nanocoating of the biological material by nano- thin shell for immunoisolation including the artificial pancreas, also analysis of the influence of factors produced by microorganisms on eukaryotic cells.
Our present work cover construction of the systems for anticancer factor delivery purposes. Moreover, the membrane systems for controlled cell growth are developed.
The confocal microscopy, atomic force microscopy, spectroscopy, electron microscopy and flow cytometry are utilized in our research.

Keywords: bioencapsulation, membrane, nanocoating, immunoisolation, biocompatibility, anticancer therapy

Publication:

1. 1. A. Kwiatkowska, M. Drabik, A. Lipko, A. Grzeczkowicz, R. Stachowiak, A. Marszalik, L.H. Granicka. Composite Membrane Dressings System with Metallic Nanoparticles as an Antibacterial Factor in Wound Healing; Membranes (Basel), Review; (2022) 13;12(2):215 doi: 10.3390/membranes12020215
   2. Anna Grzeczkowicz, Angelika Kwiatkowska, Monika Drabik, Ludomira Granicka: Nanoencapsulation of neuronal cells for cryopreservation purposes. Desalination and Water Treatment, (2021) 1-11; doi: 10.5004/dwt.2021.26655.
   3. Monika Drabik, Anna Grzeczkowicz, Paweł Bącal, Angelika Kwiatkowska, Marcin Strawski, Magdalena Antosiak-Iwańska, Beata Kazimierczak, Ewa Godlewska and Ludomira H. Granicka: Nanocomposite Membrane Scaffolds for Cell Function Maintaining for Biomedical Purposes. Nanomaterials, (2021), 11, 1094. https://doi.org/10.3390/nano11051094.
   4. A. Grzeczkowicz, M. Drabik, A. Lipko, P. Bącal, A. Kwiatkowska, Kazimierczak B., and L.H. Granicka: A Composite Membrane System with Gold Nanoparticles, Hydroxyapatite, and Fullerenol for Dual Interaction for Biomedical Purposes. Membranes, (2021), 11(8): 565. https://doi.org/10.3390/membranes11080565.
   5. Drabik, M.; Grzeczkowicz, A.; Bącal, P.; Kwiatkowska, A.;Strawski, M.; Antosiak-Iwańska, M.;Kazimierczak, B.; Godlewska, E.;Granicka, L.H. Nanocomposite Membrane Scaffolds for Cell Function Maintaining for Biomedical Purposes. Nanomaterials 2021, 11,1094. https://doi.org/10.3390/nano11051094
   6. A. Grzeczkowicz, A. Kwiatkowska, M. Drabik, L. Granicka Nanoencapsulation of neuronal cells for cryopreservation purposes. Desalination and Water Treatment 214 (2021) 135–145
   7. M. Antosiak-Iwańska, P. Bącal, B. Kazimierczak, A. Kwiatkowska, E. Godlewska, A. Grzeczkowicz, R. Stachowiak, J. Bielecki, L.H. Granicka. Polyelectrolyte Membrane with Hydroxyapatite and Silver Nanoparticles as a Material for Modern Wound Dressings. J Biomed Nanotechnol. 2020 May 1;16(5):702-714. doi: 10.1166/jbn.2020.2907.
   8. Cezary Wojciechowski, Magdalena Mazurek-Budzynska, Anna Palinska, Andrzej Chwojnowski, Ludomira Granicka, Wioleta Sikorska, Gabriel Rokicki: Preparation and characterization of partially degradable hollow fiber membranes based on polysulfone/poly(L-lactide-co-glycolide-co-e-caprolactone) blends. Desalination and Water Treatment 202 (2020)38-47; doi: https://doi.org/10.5004/dwt.2020.26154.
   9. Anna Trusek, Edward Kijak, Ludomira Granicka: Graphene oxide as a potential drug carrier – chemical carrier activation, drug attachment and its enzymatic release. Materials Science & Engineering C, (2020),116:111240; https://doi.org/ 10.1016/j.msec.2020.111240.
      4. Lucja Dybowska-Sarapuk, Weronika Sosnowicz, Jakub Krzeminski, Anna Grzeczkowicz, Ludomira H. Granicka, Andrzej Kotela and Malgorzata Jakubowska: Printed Graphene Layer as a Base for Cell Electrostimulation—Preliminary Results. Int. J. Mol. Sci., (2020), 21(21), 7865; doi:10.3390/ijms21217865.
   10. A. Grzeczkowicz, J. Gruszczyńska-Biegala, M. Czeredys, A. Kwiatkowska, M. Strawski, M. Szklarczyk, M. Koźbiał, J. Kuźnicki, L. H. Granicka: Polyelectrolyte membrane scaffold sustains growth of neuronal cells. J Biomed Mater Res Part A, 107(4):839-850; 2019
   11. A. Kwiatkowska, L. H. Granicka, A. Grzeczkowicz, R. Stachowiak, P. Bącal, K. Sobczak, M. Darowski, M. Kozarski, J. Bielecki: Gold nanoparticle-modified poly(vinyl chloride) surface with improved antimicrobial properties for medical devices. J. Biomed. Nanotechnol. 14: 922-932; 2018
   12. A. Kwiatkowska, L. H. Granicka, A. Grzeczkowicz, R. Stachowiak, M. Kamiński, Z. Grubek, J. Bielecki, M. Strawski, M. Szklarczyk: Stabilized nanosystem of nanocarriers with an immobilized biological factor for anti-tumor therapy. PlosOne,doi. 10.1371/ journal.pone.0170925; 2017.
   13. Marcin Strawski, Ludomira H. Granicka, Marek Szklarczyk: Redox properties of polyelectrolyte multilayer modified electrodes: a significant effect of the interactions between the polyelectrolyte layers in the films. Electrochimica Acta 226: 121–131; 2017.
   14. A. Kwiatkowska, L. Granicka, A. Grzeczkowicz, A. Chwojnowski, C. Wojciechowski, Nano-thin polielectrolytes layers for biotechnological applications. Desalination and Water Treatment. 64: 260-265; 2017.
   15. A. Grzeczkowicz, L. H. Granicka, I. Maciejewska, M. Strawski, M. Szklarczyk, M. Borkowska: The experimental study of the performance of nano-thin polyelectrolyte shell for dental pulp stem cells immobilization. J. Nanosci. Nanotechnol. 15, 9531-9538; 2015.
   16. Alicja Mackiewicz, Anna Grzeczkowicz, Ludomira Granicka, Magdalena Antosiak-Iwańska, Ewa Godlewska, Dariusz Gozdowski, Dorota Olczak-Kowalczyk: Cytotoksyczność materiału Nanocare Gold® w badaniu in vitro – badanie pilotażowe (Cytotoxicity of Nanocare Gold® in In Vitro Assay – Pilot Study). Dent. Med. Probl. 52, 2, 167–174; 2015.
   17. M. Borkowska, A. Grzeczkowicz, M. Strawski, J. Kawiak, M. Szklarczyk, L. H. Granicka: Performance and detection of nano-thin polyelectrolyte shell for cell coating. J Nanopart Res, 16 (7): art. no. 2488; 2014.
       Ludomira H. Granicka: Nanoencapsulation of Cells Within Multilayer Shells for Biomedical Applications. J Nanosci Nanotechnol, Vol. 14, 705–716, 2014.
   18. L. H. Granicka, M. Borkowska, A. Grzeczkowicz, R. Stachowiak, M. Szklarczyk, J. Bielecki, M. Strawski: The targeting nanothin polyelectrolyte shells in system with immobilized bacterial cells for antitumor factor production. J Biomed Mater Res A, 102 (8): 2662-2668; 2014.
   19. M. Bulwan, M. Antosiak-Iwanska, E. Godlewska, L. Granicka, S. Zapotoczny, M. Nowakowska: Chitosan-based nanocoatings for hypothermic storage of living cells. Macromol Biosci, 13 (11): 1610-1620; 2013.
   20. M. Borkowska, E. Godlewska, M. Antosiak-Iwańska, J. Kinasiewicz, M. Strawski, M. Szklarczyk and L. H. Granicka: Suitability of polyelectrolyte shells modyfied with fullerene derivate for immunoisolation of cells. Experimental study. J Biomed Nanotechnol, 8(6):1-6; 2012.

Patents

1. L. Granicka, A. Grzeczkowicz, A. Kwiatkowska: Układ membranowy do miejscowej ukierunkowanej immobilizacji komórek oraz sposób jego wytwarzania, Patent nr 236035; 2020.
2. L. Granicka, A. Grzeczkowicz, M. Borkowska: Układ membranowy do miejscowej immobilizacji komórek oraz sposób jego wytwarzania. Patent nr P-403498; 2016.
3. L. Granicka, J. Kawiak, R. Stachowiak, J. Wiśniewski, J. Bielecki, M. Łyżniak: "Membrana poliolefinowa do izolacji mikroorganizmów Gram +". Patent nr P-389847; 2012.