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Laboratory of Biosensors and Analytical Microsystems

Joanna Jankowska-Śliwińska, Ph.D Eng. - Head of The Laboratory
Władysław Torbicz, Ph.D Eng. Prof.
Dorota G. Pijanowska, Ph.D Eng. Assoc. Prof.
Marek Dawgul, Ph.D, Eng.
Konrad Dudziński, Ph.D Eng.
Elżbieta Remiszewska, MSc
Anna Baraniecka, MSc
Agnieszka Paziewska-Nowak, MSc Eng.
Anna Marczak, Eng.
Jerzy Kruk, constructor
Tomasz Raczyński

RESEARCH

Biosensors and microsystems for multi-parametric biochemical analysis

High demand for medical diagnostics improvement in the field of analysis of saliva, plasma or urine of hospitalized patients or people treated at home has led to a growing need for the development and manufacturing of miniature analytical devices, such as: biosensors and flow analytical microsystems.

Biosensors are used for the determination of individual substances and/or monitoring of their concentration. They can be also combined in matrices for a multi-parametric assays. In turn, flow analytical microsystems are used for simultaneous identification of several biochemical compounds and can be utilized in “point-of-care” tests. Biosensors can be used for determination of both: substances naturally occurring in body fluids, such as glucose, electrolytes (Na+, K+, Ca2+, Mg2+, Cl-) and substances of endogenous origin, like: proteins (including enzymes and hormones), various metabolites (e.g.: urea, creatinine, lactates, etc.) as well as therapeutics such as anticancer drugs or antidepressants. Moreover, biosensors can be designed and successfully used for selective determination of various types of toxins
e.g. pesticides, herbicides, heavy metal ions. Important advantages of biosensors are: short response time, reliability, possibility of measurements in low volume samples, low cost of production which results in reduction of the cost of single analysis. Very important parameters influencing the quality of the results obtained with the use of biosensors are: sensitivity, selectivity and stability.

In Laboratory of Biosensors and Analytical Microsystems, we are focused on development of biosensors and flow analytical microsystems utilized both: an optical and electrochemical detection.

Our previous research on biosensors concerned the development of: sensors’ structures (chemical modification of the surface, immobilization of bioreceptors) for determination of tricyclic antidepressant drugs, immunosensors for detection of human C-reactive protein (CRP) and troponin I, sensors for determination of psychoactive compounds based on the intercalation phenomena and enzymatic biosensors containing lactate dehydrogenase and NAD+ as a receptor for the determination of lactates concentration.

Currently, we are developing biosensors based on conductive polymers and graphene, which are dedicated to the various biochemical analytes, e.g.: urea, lactates, dopamine and isatin.

keyenceMeasurements and imgages performed using the VHX-6000 microscope through the courtesy of Keyence International NV/SA company.

Very important part of our work is based on research related to elaboration of integrated flow microsystems containing: enzymatic microreactor, mixer and flow cell with optical detection. These systems are manufactured with the use of low-temperature co-fired ceramic (LTCC) technology or micromechanics of silicon. Additionally, various methods of functionalization and bioreceptor immobilization are used for different surfaces modification (glass, silicon, ceramics, polymers).

mikrobioreaktory ceramiczneCeramic microbioreactors

LABORATORY EQUIPMENT:

We have three laboratories:

  1. Technological laboratory is equipped with devices for the application of various types of layers and the manufacturing of sensor structures, such as Ultra TT (EFD) micro-dosing robot for direct printing method and semiautomatic screen printer which was constructed in IBIB PAN and a plasma chamber (Harrick Plasma).
  2. In the surface analysis laboratory there is a goniometer (DSA 25, KRŰSS), two Olympus stereoscopic microscopes SZX9 and SZ51, an FTIR spectrometer (Excalibur 3000, BioRAD) equipped with a Raman adapter (Varian)
  3. The analytical laboratory is equipped with: single-channel surface plasmon resonance spectrometer (Springle, KE Instruments) with an electrochemical call, stationary potentiostat (VMP2 / Z, PAR BioLogic), several portable potentiostat (PalmSens, Palm Instruments BV), Solartron SI 1287 impedance spectrometry analyzer with SI 1260 electrochemical insert, spectrophotometers: plate Synergy HT (BioTek) and S.I. UV-Vis spectrophotometer Photonics 440 with the immersion probe, reflective probe and flow cell, high performance liquid chromatography system (Varian).

folia czujnikiPoliester foil of 96 sensors printed with the use of Ultra TT (EFD) microdosing robot and exemplary cyclic voltammogram obtained after measurements performed in Fe(CN)64- solution.

DEFENDED PhDs:

  1. Joanna Jankowska-Śliwińska: „Electrochemical detection of psychoactive substances based on intercalation to DNA”. Promoter: Assoc. Prof. PhD Eng. Dorota Pijanowska (obtaining a doctorate in technical sciences in discipline of biocybernetics and biomedical engineering, 2016)
  2. Rafał Szczypiński: „Moduł mikroprzepływowy do pomiarów cytometrycznych”. Promoter: Assoc. Prof. PhD Eng. Dorota Pijanowska (obtaining a doctorate in technical sciences in discipline of biocybernetics and biomedical engineering, 2016)
  3. Beata Kazimierczak: „Amperometryczne immunoczujniki do oznaczeń wybranych markerów chorób układu krążenia”. Promoter: Assoc. Prof. PhD Eng. Dorota Pijanowska (obtaining a doctorate in technical sciences in discipline of biocybernetics and biomedical engineering, 2015)

List of publications:

  1. K. Dudziński, M. Dawgul, K. Pluta, B. Wawro, W. Torbicz, D.G. Pijanowska, Spiral concentric two electrode sensor fabricated by direct writing for skin impedance measurements, IEEE Sens. J., 2017, 17, 5306-5314.
  2. A. Paziewska-Nowak, J. Jankowska-Śliwińska, M. Dawgul, D.G. Pijanowska, Selective electrochemical detection of pirarubicin by means of DNA-modified graphite biosensors, Electroanalysis, 2017, 29, 1810-1819.
  3. J. Jankowska-Śliwińska, M. Dawgul, J. Kruk, D.G. Pijanowska, Comparison of electrochemical determination of purines and pyrimidines by means of carbon, graphite and gold electrodes, Int. J. Electrochem. Sc., 2017, 12, 2329-2343.
  4. C.M. Yang, Y.H. Liao, C.H. Chen, T.C. Chen, C.S. Lai, D.G. Pijanowska, P-I-N amorphous silicon for thin-film light-addressable potentiometric sensors, Sensor. Actuat. B.-Chem., 2016, 236, 1005-1010.
  5. W.Y. Chung, A.A. Silverio, V.F.S. Tsai, C. Cheng, S.Y. Chang, Z. Ming-Ying, C.Y. Kao, S.Y. Chen, D.G. Pijanowska, D, Rustia, Y.W. Lo, An implementation of an electronic tongue system based on a multi-sensor potentiometric readout circuit with embedded calibration and temperature compensation, Microelectron. J., 2016, 57, 1-12.
  6. B. Kazimierczak, D.G. Pijanowska, A. Baraniecka, M. Dawgul, J. Kruk, W. Torbicz, Immunosensors for human cardiac troponins and CRP, in particular amperometric cTnI immunosensor, Biocybern. Biomed. Eng., 2016, 36, 29-41.
  7. K. Malecha, E. Remiszewska, D.G. Pijanowska, Technology and application of the LTCC-based microfluidic module for urea determination, Microelectron. Int., 2015, 32, 126-132.
  8. C.M. Yang, T.F. Lu, K.I. Ho, J.C. Wang, D.G. Pijanowska, B. Jaroszewicz, C.S. Lai, HfOxFy Based ISFETs with Reactive Fluorine Doping for K+ ion Detection, Int. J. Electrochem. Sc., 2014, 9, 7069-7082.
  9. K .Malecha, E. Remiszewska, D.G. Pijanowska, Surface modification of low and high temperature co-fired ceramics for enzymatic microreactor fabrication, Sensor. Actuat. B.-Chem., 2014, 190, 873-880.
  10. C.M. Yang, I.S. Wang, Y.T. Lin, C.H. Huang, T.F. Lu, C.E. Lue, D.G. Pijanowska, M.Y. Hua, C.S. Lai, Low Cost and Flexible Electrodes with NH3 Plasma Treatments in Extended Gate Field Effect Transistors for Urea Detection, Sensor. Actuat. B.-Chem., 2013, 187, 274– 279.
  11. J.H. Yang, T.F. Lu, J.C. Wang, C.M. Yang, D.G. Pijanowska, C.H. Chin, C.E. Lue, C.S. Lai, LAPS with nanoscaled and highly polarized HfO2 by CF4 plasma for NH4(+) detection, Sensor. Actuat. B.-Chem., 2013, 180, 71-76.
  12. C. Cheng, K.C. Chang, D.G. Pijanowska, On-line flow injection analysis using gold particle modified carbon electrode amperometric detection for real-time determination of glucose in immobilized enzyme hydrolysate of waste bamboo chopsticks, J. Electroanal. Chem., 2012, 666, 32-41.
  13. I.S. Wang, Y.T. Lin, C.H. Huang, T.F. Lu ,C.E. Lue, P. Yang, D.G. Pijanowska, C.M. Yang, J.C. Wang, J.S. Yu, Y.S. Chang, C. Chou, C.S. Lai, Immobilization of enzyme and antibody on ALD-HfO2-EIS structure by NH3 plasma treatment, Nanoscale Res. Lett., 2012, 7, 179.
  14. K. Malecha, M. Dawgul, D.G. Pijanowska, L.J. Golonka, LTCC microfluidic systems for biochemical diagnosis, Biocybern. Biomed. Eng., 2011, 31, 31-41.
  15. D.G. Pijanowska, A. Kossakowska, W. Torbicz, Electroconducting polymers in (bio)chemical sensors, Biocybern. Biomed. Eng., 2011, 31, 43-51.
  16. K Malecha, D.G. Pijanowska, L.J. Golonka, P. Kurek, Low temperature co-fired ceramic (LTCC)-based biosensor for continuous glucose monitoring,
    Sensor. Actuat. B.-Chem., 2011, 155, 923-929.
  17. T.F. Lu, C.M. Yang, J.C. Wang, K.I. Ho, C.H. Chin, D.G. Pijanowska, B. Jaroszewicz, C.S. Lai, Characterization of K+ and Na+-sensitive membrane fabricated by CF4 plasma treatment on hafnium oxide thin films on ISFET, J. Electrochem. Soc., 2011, 158, J91-J96.
  18. C.S. Lai, T.F. Lu, C.M. Yang, Y.C. Lin, D.G. Pijanowska, B. Jaroszewicz, Body effect minimization using single layer structure for pH-ISFET applications, Sensor. Actuat. B.-Chem, 2010, 143, 494-499.
  19. K. Malecha, D.G. Pijanowska, L.J. Golonka, W. Torbicz, LTCC microreactor for urea determination in biological fluids, Sensor. Actuat. B.-Chem, 2009, 141, 301-308.
  20. C.S. Lai, C.E. Lue, C.M. Yang, M. Dawgul, D.G. Pijanowska, Optimization of a PVC Membrane for Reference Field Effect Transistors, Sensors, 2009, 9, 2076-2087.

Patents:

  1. W.Y. Chung, T.T. Kuo, Y.H. Wang, D.G. Pijanowska, W. Torbicz: Signal readout circuit for amperometric sensor, US Patent Pending, 2008.
  2. W.Y. Chung, C.H. Yang, D.G. Pijanowska, P. Grabiec, B. Jaroszewicz, W. Torbicz: Electronic circuit for ion sensor with body effect reduction, US Patent, US 7,368,917 B2, 2008.
  3. J.M. Łysko, D.G. Pijanowska: Semiconductor-type ion-selective sensor (Półprzewodnikowy czujnik jonoselektywny), Patent Pending PL347141, 2007.
  4. J.M. Łysko, D.G. Pijanowska, E. Malinowska, J. Jaźwiński: Ion-selective silicon electrode (Krzemowa elektroda jonoselektywna), Polish Patent Pl193862, 2007.
  5. W.Y Jung, A. Krzysków, Y.T. Lin, D.G. Pijanowska, J.H. Yang: Ion-sensitive circuit, Taiwanese Patent, TW235236b, 2005.
  6. W.Y. Chung, A. Krzyśków, Y.T. Lin, D.G. Pijanowska, C.H. Yang, W. Torbicz: Electronic circuit for ion sensor, US Patent US 6,906,524 B2, 2005.
  7. A. Krzyśków, D.G. Pijanowska, J. Kruk: Controller of working point for chemical sensor of ion-sensitive field effect transistor ISFET type (Regulator punktu pracy czujnika chemicznego typu jonoczuły tranzystor polowy ISFET), Polish Patent Pl178242, 1996.

 

Keywords: biosensors, enzymatic biosensors, immunosensors, DNA-based biosensors, ISFET, ChemFET, EnFET, microreactors, surface modification, immobilization of bioreceptors, microanalytical systems, lab-on-a-chip.

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